学術論文・エビデンス

最終更新日:2018年4月

2017.9.22 〜 9.24 開催

第47回日本口腔インプラント学会学術大会

新規炭酸アパタイト製人工骨の開発 〜 in vivo及びin vitroにおける吸収特性の評価 〜

重光勇介、山中克之

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2015.01 公開

Dental Materials Journal 2015;34(1):78-85

Seeding of mesenchymal stem cells into inner part of interconnected porous biodegradable scaffold by a new method with a filter paper

Katsuyuki YAMANAKA,Katsushi YAMAMOTO,Yuhiro SAKAI,Youko SUDA,Yusuke SHIGEMITSU,Tadashi KANEKO,Koichi KATO,Tomohiro KUMAGAI and Yukio KATO

An appropriate physical support provided by scaffolds creates a supportive environment that directs proliferation and differentiation of stem cells. However, it is difficult to homogenously inoculate stem cells into the inner part of scaffolds at high cell densities. In this study, mesenchymal stem cells were seeded into a hydroxyapatite/poly (D, L-lactic-co-glycolic acid) (HAP/PLGA) scaffold that had enough mechanical strength and porous 3-D structure. With an aid of a filter paper placed under the bottom of a HAP/PLGA block, the cells suspended in a culture medium flowed from the top to the bottom through interconnected pores in the scaffold, and distributed almost homogenously, as compared to cell distribution near the surface of the block by the conventional method using centrifugation or reduced pressure. This simple method with a filter paper may be useful in preparation of cell-scaffold complexes for tissue engineering.

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2015.03.11 〜 03.14 開催

93rd IADR,Boston,U.S.A.

Bone Augmentation Using 3D Cultured Chondrocytes in different Sites

Y.SHIGEMITSU、K.YAMANAKA、Y.SAKAI、Y.SUDA、T.KUMAGAI

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